BS EN 12916-2000 Methods of test for petroleum and its products一 BS 2000-391: Petroleum products – Determination of aromatic hydrocarbon types in middle distillates – High performance liquid chromatography method with refractive index detection (Identical with IP 391-2001).
6 Apparatus
61 Uquad chromatograph consisting of a tegh performance matrument capable ci purrpng the mobile
phase at flow rates of between 0.5 mWmin end 1.3 mIlm.n, with a precelon beSer than
0,5 % and pubabon of 1 $ full scale deflection i,ider the test conditions described in clause 8
62 Sampli inecbon system, capable of infecting 10 i (nonnal) ci sample soluton with irepeaabeltyol better thai I %.
NOTE 1 Equst and constant volume. of the calibration and sample eohone we wjectod into the cliraliatograpit Both manual and automatic sample inpection systems (using either complete or partial fifing of the sample leop), when used correy, can meet these repeatability reqLwemerits.
When islng the penal filing made, It Is recommended that the injection volume should be less than half the total loop volijnw. For complete filling of the loop, best resi.*s are obtained by overfihing the loop at least sax times.
The repeaIabiy of the qection system may be checked by companng peak areas from at least tour vifedlona of the system calitwon standard (see 83).
NOTE 2 Sample aid calibration lrecbon volumes daibrent froni 10 tl (typicaly it lie range 3 i to 20 Ii) may be used provided they meet these requaements * rjecbcn repeatability, refractive wades sensitivity end linearity (see 9.4). end cofteran resolulion (see 8.8)
6.3 Sample tiltqr if aequired (see 101), consisang of a n’icrofllter of porostty 0.45 urn or baa, chemically wisH towards hydrocarbon solvents, for the removal of particulate matter from the sample solutions.
6.4 Column system, consisting of a stainless steel HPLC coliann(s) pedced with a oorrwnerclal 3 im. S am or 10 xn arrenobanded (or amin&cysno-bonded) sac. stationary phase meeting the resolution requserrants given in 8.6 See Mnex A for guidance on the selection and use of suitable colemin systems
6.5 Temperatur, control neisling of either a block heating, or an air-circulating, HPLC coernwi oven or an
alternative form of tenipemature control. such as a temperalur,contrclled laboratory. cupable of maintaining
a constant temperure to within *1 ‘C in the range 20 C to 40 C
NOTE The refractive wades detector is sensitive to both sudden and gradual changes in temperature ci the eluert All necessary precautions should be taken to establish constant temperature conditions throughout the liquid chrometoapli system The tenperatur, should be optimlr.d depenng on the stationary phase
6.6 Bckflush valve, consisting of a mwv.ial. or air or eleciricaly actuated automatic. flow-switching valve designed for use Ii HPLC systems, capable of operating at pressures up 102 x 10 kPa
6.7 Refractive index detector capable of being operated ov.r the r*active rides rang. 1.3 to 1.8 end giving a linear response over the calibration range wah a suitable output signal for Vie data system
NOTE I the detector has a facility for independent temperature cenfrol. at is recorrvnended to set it at Ii sani. temperature th. column oven
61 Computer or computing integrator, compatible with the refractive index datedor having a minimum sampling rate of 1 Hz and capable of peak area aid retention tens measurement It shall also have minimum facilities for poet-analysis data processing such as baseline correction and re-inteation
NOTE The ability to perform automatic peak detection and identilicebon and to calculate sample concenfrahons from peak area measiremerits a recommended, but is not essent.
61 Volumnitrtc flask. 10 iii and 100 ml capacIty, conforming to ISO 1042. grad. B or bitter
6.10 Analytical balance, capable ci weighing to the newest *00001 g.BS EN 12916 pdf download.